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KMID : 0603820060120040385
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Journal of Experimental & Biomedical Science
2006 Volume.12 No. 4 p.385 ~ p.391
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The Effect of Alpha Subunit of Go on Cell Growth
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Won Jung-Hee
Ghil Sung-Ho
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Abstract
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Heterotrimeric GTP binding proteins (G proteins) mediate signals generated by neurotransmitters and hormones Among G proteins, Go is found in a large quantity in brain and growth cone membranes of neurons. In spite of its abundance in neurons, the role of Go is not fully understood. In our previous study, we identified promyelocytic leukemia zinc finger protein (PLZF) as an interacting partner of alpha subunit of Go (Go{alpha}) and confirmed their interaction employing several biochemical assays. To date, it is reported that PLZF functioned as a cell growth suppressor and a transcription repressor. To determine effect of Go{alpha} and PLZF interaction on the cellular function of PLZF, we performed luciferase reporter gene assay and BrdU incorporation assay. Co-expression of Go{alpha} and PLZF synergistically increased the effect of PLZF alone. These results suggest that Go{alpha} may act as cellular activator of PLZF. This novel feature of Go may provide insights into understanding diverse role of Go-coupled receptor as well as its cellular actions.
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KEYWORD
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G-proteins, PLZF, Growth suppression, Cyclin A
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